Pharmigene AutoEx16 Genomic DNA Tissue Kit is designed for purification of total DNA (including genomic, mitochondrial and viral DNA) from a variety of animal tissues or cells by using AutoEx16 auto-extraction instrument. The provided Filter Column can filtrate hard tissue sample or swab sample to prevent tissue residues to obstruct pipette tip during the process of AutoEx16 . The method uses pre-filled cartridge contains proteinase K and a chaotropic salt to lysis cells and degrade protein. DNA will bind to cellulose coated magnetic beads. After washing off the contaminants, the purified DNA is eluted by low salt elution buffer. Purified DNA of approximately 20-30 kb in length is suitable for PCR or other enzymatic reactions.
Using magnetic-particle technology to purify genomic DNA from animal tissues, paraffin embedded tissue, swab and blood stain. The purified genomic DNA can be directly used for downstream application such as quantitative PCR, restriction enzyme digestion, southern blotting... etc.
Storage and Stability:
1. This kit should be stored at room temperature.
2. Proteinase K should be stored at -20°C when mixing with PK Storage Buffer.
3. Shelf life 12 months